GCSF-PEG ADA ELISA kit | EL-141-73196–GCSF-PEG-ADA
Cat. No: EL-141-73196--GCSF-PEG-ADA
$859.99 USD
96 wells
| Target protein | PEG-GCSF (Granulocyte colony -stimulating factor) |
|---|---|
| Alternative name | PEG-filgrastim |
| Application | Measure anti-PEG-GCSF antibodies |
| Reactivity | human |
| Detection range | 45 ng/mL -1440 ng/mL |
| Sample type | Serum Plasma |
| Sample volume | 15ul |
| Assay time | 2.5 hours |
| Precision | <20%, <20% |
| Components | Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3 |
| Storage | Stable at -20C for 1 year |
Introduction
The AffinityImmuno GCSF-PEG ADA ELISA kit contains the components required for measuring antibodies to GCSF-PEG in biological matrices such as human serum, plasma or cell culture.
Principal of the Essay
This assay employs the indirect enzyme immunoassay technique. GCSF-PEG is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF-PEG present in biological matrices are bound to the immobilized GCSF-PEG. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development is proportional to the amount of antibodies to GCSF- PEG present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.
Related assay
Measuring drug levels rather than immunogenicity? See the GCSF-PEG pharmacokinetic ELISA kit.