Introduction

The AffinityImmuno GCSF-PEG ADA ELISA kit contains the components required for measuring antibodies to GCSF-PEG in biological matrices such as human serum, plasma or cell culture.

Principal of the Essay

This assay employs the indirect enzyme immunoassay technique. GCSF-PEG is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF-PEG present in biological matrices are bound to the immobilized GCSF-PEG. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development is proportional to the amount of antibodies to GCSF- PEG present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.