GCSF-PEG ADA ELISA kit | EL-141-73196–GCSF-PEG-ADA
Cat. No: EL-141-73196--GCSF-PEG-ADA
$859.99 USD
96 wells
| Target protein | PEG-GCSF (Granulocyte colony -stimulating factor) |
|---|---|
| Alternative name | PEG-filgrastim |
| Application | Measure anti-PEG-GCSF antibodies |
| Reactivity | human |
| Detection range | 45 ng/mL -1440 ng/mL |
| Sample type | Serum Plasma |
| Sample volume | 15ul |
| Assay time | 2.5 hours |
| Precision | <20%, <20% |
| Components | Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3 |
| Storage | Stable at -20C for 1 year |
Introduction
The AffinityImmuno GCSF-PEG ADA ELISA kit contains the components required for measuring antibodies to GCSF-PEG in biological matrices such as human serum, plasma or cell culture.
Principal of the Essay
This assay employs the indirect enzyme immunoassay technique. GCSF-PEG is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF-PEG present in biological matrices are bound to the immobilized GCSF-PEG. After washing away any unbound substances, antibody-enzyme reagent and a substrate solution is added to the wells for color development is proportional to the amount of antibodies to GCSF- PEG present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.