Introduction

The AffinityImmuno GCSF-PEG (Pegylated Filgrastim) ELISA kit contains the components required for measuring natural and recombinant human GCSF-PEG in biological matrices such as human serum, plasma, or cell culture supernatant.

Principal of the Essay

This assay employs the indirect sandwich enzyme immunoassay technique. Anti -GCSF-PEG is coated onto 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. GCSF-PEG present in biological matrices is bound by the immobilized anti-GCSF-PEG antibody. After washing away any unbound substances, tagged anti-GCSF-PEG secondary antibody is added to the wells. After washing diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate is added to the wells for color development. The color development is proportional to the amount of GCSF-PEG present in the test samples. The color development is stopped and the intensity of the color is measured.