Free calculators for ELISA assay development and validation. Everything below runs in your browser — no data is uploaded or stored anywhere.
4PL Curve Fitting Tool
Fit a four-parameter logistic (4PL) curve to your ELISA standard curve data, then back-calculate unknown sample concentrations from measured OD values.
ELISA Validation Suite
Run the core calculations behind an ELISA validation report: precision (intra-/inter-assay %CV), accuracy (spike-recovery), linearity of dilution, and LOD/LOQ.