Free Bioanalytical Tools for ELISA Development
Free calculators for ELISA assay development and validation. Everything below runs in your browser — no data is uploaded or stored anywhere.
4PL Curve Fitting Tool
Fit a four-parameter logistic (4PL) curve to your ELISA standard curve data, then back-calculate unknown sample concentrations from measured OD values.
Validation Suite
FDA Bioanalytical Guidance (2018) ligand-binding-assay validation calculators — accuracy & precision, LLOQ, dilutional linearity, selectivity, specificity, and prozone/hook-effect checks, each built on the same shared 4PL curve engine. Everything runs in your browser. More sections beyond Accuracy & Precision are coming, using the same pattern.