hGH ADA (human growth hormone) ELISA | EL-141-878
Cat. No: EL-141-878
$859.99 USD
96 wells
| Target protein | hGH (human growth hormone) |
|---|---|
| Alternative name | Somatropin |
| Application | Measure anti-hGH antibodies |
| Reactivity | human |
| Detection range | 30 ng/mL -1920 ng/mL |
| Sample type | Serum Plasma |
| Sample volume | 15ul |
| Assay time | 2.5 hours |
| Precision | Intra-assay coefficient variation (CV) <20%, inter-assay CV <20% |
| Components | Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 μl TMB 12 ml TMB stop solution |
| Storage | Stable at -20°C |
Introduction
The AffinityImmuno hGH (Human Growth Hormone) ADA ELISA kit contains the components required for measuring antibodies to hGH in biological matrices such as human serum, plasma or cell culture supernatant.
Principal of the assay
This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibodies
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in test samples. The color development is stopped and the intensity of the color is measured.