Introduction

The AffinityImmuno hGH (Human Growth Hormone) ADA ELISA kit contains the components required for measuring antibodies to hGH in biological matrices such as human serum, plasma or cell culture supernatant.

Principal of the assay

This assay employs the indirect enzyme immunoassay technique. hGH is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to hGH present in biological matrices are bound to the immobilized hGH. After washing away any unbound substances, antibodies
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of antibodies to hGH present in test samples. The color development is stopped and the intensity of the color is measured.