$599.99

Trastuzumab(Herceptin®) Immunogenicity ELISA
Catalog EL-141-201

Product Properties

Detection method

Peroxidase / OD450 

Storage and stability

Stable at -20°C for 1 year 

Safety Warnings and Precautions

Research Use Only. Follow instructions. 

Precision

Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. 

Detection limit

31.3ng/ml 

Each kit includes:

Coated microtiter plate, 96 wells
QC samples - 4x50ul
10X wash buffer - 25ml
Assay buffer - 50ml
1000X secondary antibody - 17ul
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3 

Category:

Description

INTRODUCTION
Trastuzumab (Herceptin® ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2). Repeated dosing of Trastuzumab has been reported to induce a neutralizing antibody response resulting in reduce efficacy of Trastuzumab. This kit allows
researchers to qualitatively monitor the presence of anti-Trastuzumab antibodies in biological matrices.
PRINCIPLE OF THE ASSAY
This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount of anti-Trastuzumab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Trastuzumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.

 

Reviews

  1. ClarissaCarr

    2 thumbs up!

Add a review

Your email address will not be published. Required fields are marked *