$599.99

Rituximab(Rituxan®) Immunogenicity ELISA
Catalog EL-141-181

Product Properties

Detection method

Peroxidase / OD450 

Storage and stability

Stable at -20°C for 1 year 

Safety Warnings and Precautions

Research Use Only. Follow instructions. 

Precision

Intra-assay coefficient of variation (CV) ranges from 4% CV to 14%CV. Inter-assay CV was 13%. 

Detection limit

7.8ng/ml 

Each kit includes:

Coated microtiter plate, 96 wells
QC samples - 4x50ul
10X wash buffer - 25ml
Assay buffer - 50ml
1000X secondary antibody - 17ul
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3 

Category:

Description

INTRODUCTION
Rituximab (Rituxan® ) is a chimeric mouse/human monoclonal antibody used for treating B cell malignancy, autoimmune conditions, and graft rejection by depleting B cells from the body. This immunogenicity assay employs the bridging ELISA technique. Four levels of QC samples give a qualitative reference signal which can be used to determine the level of anti- Rituximab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.
PRINCIPLE OF THE ASSAY
This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Rituximab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount of anti-Rituximab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Rituxumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.

 

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Catalog EL-141-181”

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