Human PD-L2 ELISA Kit | EL-213-16402
Cat. No: EL-213-16402
$859.99 USD
96 wells
| Target protein | PD-L2 |
|---|---|
| Alternative name | PDCD1LG2 |
| Gene ID | 80380 |
| Reactivity | Human |
| Detection range | 0.16-10 ng/mL |
| Sample type | tissue homogenates, cell lysates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay to quantify human PD-L2 (PDCD1LG2) in biological samples.
Introduction
PD-L2, encoded by the PDCD1LG2 gene, is a roughly 31 kDa transmembrane immunoglobulin-family protein built from an Ig-like V-type domain and a C2-type domain anchored by a short cytoplasmic tail. Alongside PD-L1, it serves as one of two known ligands for the checkpoint receptor PD-1 on T cells, and its engagement dampens T-cell proliferation, cytokine output, cytolytic activity, and cell survival, pushing chronically stimulated T cells toward an exhausted state. Through this mechanism PD-L2 contributes to maintaining tolerance toward self-antigens and has been implicated in immune privilege during pregnancy, graft acceptance, autoimmune conditions, and tumor immune evasion, while elevated expression has also been reported as a marker in pulmonary tuberculosis. Because a soluble form circulates in blood, sensitive sandwich immunoassays have been developed to quantify PD-L2 as a companion readout alongside PD-1 and PD-L1 in immune-monitoring studies.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to human PD-L2 (PDCD1LG2). Standards or samples are pipetted into the appropriate wells together with a biotin-conjugated detection antibody that also recognizes human PD-L2. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate. Upon addition of TMB substrate solution, a colorimetric signal develops exclusively in wells where human PD-L2, the biotin-conjugated detection antibody, and the avidin-HRP conjugate are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid stop solution, and absorbance is measured spectrophotometrically at 450 nm (± 10 nm). Sample concentrations are derived by interpolating the measured optical densities against a standard curve.