Human IL-37 ELISA Kit | EL-213-91237
Cat. No: EL-213-91237
$859.99 USD
96 wells
| Target protein | IL-37 |
|---|---|
| Gene ID | 27178 |
| Reactivity | Human |
| Detection range | 7.82-500 pg/mL |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human IL-37 in biological samples.
Introduction
IL-37, a member of the IL-1 cytokine family, functions as one of the body’s few naturally anti-inflammatory interleukins, acting to restrain rather than amplify innate immune activation. It can act through two distinct routes: after translocating into the nucleus together with SMAD3, it can directly dampen inflammatory gene transcription from within immune cells, and after being secreted, it engages a cell-surface receptor complex that opposes normal IL-18 signaling. Through these pathways IL-37 lowers production of numerous pro-inflammatory mediators, including IL-1-family cytokines, IL-6, and several chemokines, while leaving anti-inflammatory cytokine output largely intact, and it also dampens dendritic cell activation. Several naturally occurring isoforms of IL-37 have been described, with the most complete isoform being the most extensively studied form of the cytokine, and it is expressed at low baseline levels in tissues such as kidney, thymus, and monocytes, consistent with a role in keeping everyday inflammatory tone in check.
Principle of the Assay
This kit utilizes a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human IL-37. Standards or samples are introduced into the appropriate wells along with a biotin-conjugated detection antibody that recognizes a second epitope on Human IL-37. Avidin conjugated to Horseradish Peroxidase (HRP) is then added to each well and allowed to incubate, linking enzymatic activity to captured analyte. Upon addition of TMB substrate solution, a colorimetric signal develops exclusively in wells where Human IL-37, the biotin-conjugated antibody, and Avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid stop solution, and the resulting color intensity is measured spectrophotometrically at 450 nm (± 10 nm). Sample IL-37 concentrations are calculated by comparing the optical density of each sample against a reference standard curve.