Human IL-36alpha ELISA Kit | EL-213-912362
Cat. No: EL-213-912362
$859.99 USD
96 wells
| Target protein | IL-36alpha |
|---|---|
| Alternative name | IL36A |
| Gene ID | 27179 |
| Reactivity | Human |
| Detection range | 7.82-500 pg/mL |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify IL-36alpha in biological samples.
Introduction
IL-36 alpha, formerly designated IL-1F6 or interleukin-1 epsilon, is a member of the interleukin-1 cytokine family that signals through the IL-36 receptor complex, pairing the receptor chain IL1RL2 with the shared accessory protein IL1RAP to activate NF-kB and MAPK cascades. Produced mainly by keratinocytes and immune cells within skin and other epithelial barriers, it drives local inflammation by inducing neutrophil-, monocyte-, and T-cell-attracting chemokines and by promoting dendritic cell maturation, effects that amplify pro-inflammatory feedback loops. Its expression rises markedly in psoriatic skin lesions, and IL-36 alpha is studied as a contributor to psoriasis and other chronic inflammatory skin and bowel conditions. Altered expression of the gene has also been reported in some solid tumors, where lower levels have been associated with worse outcomes in hepatocellular and colorectal cancer, suggesting a role beyond classical barrier immunity that is still being defined.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format. Microtiter plate wells are pre-coated with a capture antibody specific to Human IL-36alpha (IL-1ε). During the assay, standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody that also recognizes Human IL-36alpha. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate. Upon addition of the TMB substrate solution, a colorimetric signal develops exclusively in wells where Human IL-36alpha, the biotin-conjugated detection antibody, and avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid stop solution, and absorbance is read spectrophotometrically at 450 nm (± 10 nm). Sample concentrations are determined by comparing sample optical density values against a standard curve.