Human IL-18BP ELISA Kit | EL-213-91218
Cat. No: EL-213-91218
$859.99 USD
96 wells
| Target protein | IL-18BP |
|---|---|
| Gene ID | 10068 |
| Reactivity | Human |
| Detection range | 1.57-100 ng/mL |
| Sample type | Serum, plasma and other biological fluids. |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify IL-18BP in biological samples.
Introduction
IL-18BP is a small, constitutively secreted protein built around a single immunoglobulin-like domain that circulates freely in blood and acts as a natural brake on interleukin-18 signaling. By binding IL-18 before it can engage its cell-surface receptor, it blunts the downstream burst of interferon-gamma and dampens the type of T-helper-1-skewed immune response that IL-18 would otherwise drive, and it may also shape type 2 (Th2) cytokine output. The protein is produced constitutively by mononuclear immune cells and is also strongly expressed in tissues such as heart, lung, spleen, and placenta, and several splice-derived isoforms of differing size exist, though only one has been shown to bind IL-18 effectively. Altered levels of IL-18BP have been reported in inflammatory bowel conditions such as Crohn’s disease, and rare inherited defects in the gene have been linked to a severe, recessive form of fulminant viral hepatitis.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format to measure Human IL-18BP. The microtiter plate wells are pre-coated with a capture antibody specific to Human IL-18BP, which binds the target analyte present in standards or samples upon addition to the wells. A biotin-conjugated detection antibody, also specific to Human IL-18BP, is then introduced and recognizes a second epitope on the captured analyte. Avidin conjugated to horseradish peroxidase (HRP) is subsequently added to each well, where it binds the biotin moiety of the detection antibody. Following addition of TMB substrate solution, only wells containing Human IL-18BP, the biotin-conjugated detection antibody, and avidin-HRP undergo a colorimetric change. The enzymatic reaction is halted by the addition of a sulphuric acid stop solution, and absorbance is measured spectrophotometrically at 450 nm (± 10 nm). IL-18BP concentrations in unknown samples are determined by interpolating their optical density values against a standard curve.