Human IL-17F ELISA Kit | EL-213-91217
Cat. No: EL-213-91217
$859.99 USD
96 wells
| Target protein | IL-17F |
|---|---|
| Gene ID | 112744 |
| Reactivity | Human |
| Detection range | 7.82-500 pg/mL |
| Sample type | serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human IL-17F in biological samples.
Introduction
IL-17F is an inflammatory cytokine most closely associated with the Th17 lineage of T-helper cells, although it is also produced by CD8+ T cells, NK cells, mast cells, and other innate lymphoid populations. It signals largely as a heterodimer with IL-17A through a shared IL-17RA/IL-17RC receptor complex, though it can also act as a homodimer via IL-17RC alone, triggering NF-kB and MAPK signaling that drives production of neutrophil-recruiting chemokines, antimicrobial peptides, and matrix-degrading enzymes. Through this pathway it recruits and activates neutrophils to help clear extracellular bacteria and fungi at epithelial surfaces, and it further protects mucosal barriers by prompting epithelial cells to produce defensin peptides. Beyond direct antimicrobial defense, IL-17F has been reported to restrain new blood vessel growth in endothelial cells even as it stimulates their output of other inflammatory mediators, and dysregulated IL-17F/IL-17A activity is linked to chronic inflammatory conditions including psoriasis and rheumatoid arthritis.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human IL-17F. Standards or samples are added to the appropriate wells along with a biotin-conjugated detection antibody that also recognizes Human IL-17F. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate, binding to the biotin on the detection antibody. Upon addition of TMB substrate solution, a colorimetric signal develops exclusively in wells where Human IL-17F, the biotin-conjugated antibody, and avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid solution, and the resulting color is measured spectrophotometrically at 450 nm (± 10 nm). Sample concentrations are determined by comparing the absorbance readings of each sample against a standard curve generated from the provided standards.