INTRODUCTION

Somatropin (human growth hormone (hGH)) is a peptide hormone that is synthesized and secreted by the somatotropic cells of the anterior pituitary gland. Somatropin injection is used to replace growth hormone (a natural hormone produced by your body) in adults and children with growth hormone deficiency. The hGH ELISA kit is designed to measure free somat with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of hGH in biological matrices.

PRINCIPLE OF THE ASSAY

This assay employs the indirect sandwich enzyme immunoassay technique. Anti-GH is coated onto a 96 well microplate. Calibrator and test samples prepared by dilution into assay buffer and are pipetted into the appropriate wells. GH present in biological matrices is bound by the immobilized anti- GH antibody. After washing away any unbound substances, tagged anti-GH secondary antibody is added to the wells. After washing, diluted detection reagent is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of GH present in test samples. The color development is stopped and the intensity of the color is measured.