GCSF ADA (granulocyte colony stimulating factor) ELISA | EL-141-73196
Cat. No: EL-141-73196
$859.99 USD
96 wells
| Target protein | GCSF (granulocyte colony stimulating factor) |
|---|---|
| Alternative name | Filgrastim |
| Application | Measure anti-GCSF antibodies |
| Reactivity | human |
| Detection range | 45 ng/mL -1440 ng/mL |
| Sample type | Serum Plasma |
| Sample volume | 15ul |
| Assay time | 2.5 hours |
| Precision | Intra-assay coefficient variation (CV) <20%, inter-assay CV <20% |
| Components | Coated microtiter plate, 96 wells (12x8 strips) 1 Calibrator diluent 1.8 ml Calibrator (1.15mg/mL) 12 μl 10X wash buffer 50 ml Assay buffer 50 ml 1000X detection reagent 17 μl TMB 12 ml TMB stop solution |
| Storage | Stable at -20°C |
Introduction
The AffinityImmuno GCSF ADA ELISA kit contains the components required for measuring antibodies to GCSF in biological matrices such as human serum, plasma or cell culture supernatant.
Principal of the assay
This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances,
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color
development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.