Introduction

The AffinityImmuno GCSF ADA ELISA kit contains the components required for measuring antibodies to GCSF in biological matrices such as human serum, plasma or cell culture supernatant.

Principal of the assay

This assay employs the indirect enzyme immunoassay technique. GCSF is coated onto a 96 well microplate. Calibrator and test samples are prepared by dilution into assay buffer and are pipetted into the appropriate wells. Antibodies to GCSF present in biological matrices are bound to the immobilized GCSF. After washing away any unbound substances,
antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color
development is proportional to the amount of antibodies to GCSF present in the calibrator and test samples. The color development is stopped and the intensity of the color is measured.