Human CX3CL1 ELISA Kit | EL-213-32403
Cat. No: EL-213-32403
$859.99 USD
96 wells
| Target protein | CX3CL1 |
|---|---|
| Gene ID | 6376 |
| Reactivity | Human |
| Detection range | 0.16-10 ng/mL |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human CX3CL1 in biological samples.
Introduction
CX3CL1, commonly called fractalkine, is the sole human chemokine of the CX3C class and is unusual in existing both as a membrane-tethered molecule with a mucin-like stalk and, after proteolytic shedding, as a soluble mediator. Endothelial cells display the membrane-bound form following exposure to inflammatory signals such as interferon-gamma and tumor necrosis factor-alpha, allowing it to grab and firmly anchor circulating monocytes, T cells, and NK cells at the vessel wall. Once cleaved free, the soluble fragment instead acts as a classic chemoattractant, drawing monocytes and T lymphocytes toward sites of injury or inflammation through its single receptor, CX3CR1, which is concentrated on cytotoxic lymphocytes and macrophages. This chemokine-receptor pairing has been implicated in a wide array of conditions, including atherosclerosis, vasculitis, several cancers, and chronic inflammatory and neurological disorders.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format to detect Human CX3CL1. The microtiter plate wells are pre-coated with a CX3CL1-specific capture antibody, which binds the target protein present in standards or samples. A biotin-conjugated detection antibody, also specific to Human CX3CL1, is then added and binds a second epitope on the captured analyte. Avidin conjugated to horseradish peroxidase (HRP) is subsequently introduced and binds the biotin label. Upon addition of TMB substrate solution, a colorimetric signal develops only in wells where CX3CL1, the biotin-conjugated detection antibody, and the avidin-HRP conjugate are all present. The enzymatic reaction is stopped by the addition of sulphuric acid solution, and absorbance is measured at 450 nm (± 10 nm). CX3CL1 concentrations in unknown samples are determined by comparing their optical density values against a standard curve.