Human CEACAM6 ELISA Kit | EL-213-35106
Cat. No: EL-213-35106
$859.99 USD
96 wells
| Target protein | CEACAM6 |
|---|---|
| Gene ID | 4680 |
| Reactivity | Human |
| Detection range | 0.79-50 ng/mL |
| Sample type | serum, plasma, tissue homogenates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human CEACAM6 in biological samples.
Introduction
CEACAM6 (also called CD66c) is a GPI-anchored cell-surface glycoprotein belonging to the carcinoembryonic antigen family, a group of adhesion molecules that mediate both homophilic binding to themselves and heterophilic binding to related family members such as CEACAM5 and CEACAM8. On neutrophils this heterophilic interaction supports firm adhesion to cytokine-activated vascular endothelium, while in epithelial cells the same adhesive machinery has been co-opted to promote tumor cell migration, invasion, and disease progression, earning CEACAM6 recognition as an oncogenic driver in several carcinomas. Because it shares antigenic determinants with the classic tumor marker CEACAM5, elevated CEACAM6 in serum has similarly been explored as a diagnostic indicator, including in distinguishing pancreatic ductal adenocarcinoma from benign pancreatitis. The protein also serves an unrelated role as a bacterial attachment site, being exploited by adherent-invasive strains of E. coli to colonize the ileal epithelium in Crohn’s disease.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human CEACAM6. Standards or samples are pipetted into the appropriate wells alongside a biotin-conjugated detection antibody that also recognizes Human CEACAM6. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate. Upon addition of TMB substrate solution, a colorimetric signal develops only in wells where Human CEACAM6, the biotin-conjugated detection antibody, and avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid stop solution, and absorbance is measured spectrophotometrically at 450 nm (± 10 nm). The concentration of Human CEACAM6 in each sample is determined by comparing sample optical density values against a standard curve.