Human CD123 (IL-3Rα) ELISA Kit | EL-213-34123
Cat. No: EL-213-34123
$859.99 USD
96 wells
| Target protein | CD123 (IL-3Rα) |
|---|---|
| Alternative name | IL3RA |
| Gene ID | 3563 |
| Reactivity | Human |
| Detection range | 0.32-20 ng/mL |
| Sample type | serum, plasma, tissue homogenates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human CD123 (IL-3Rα) in biological samples.
Introduction
IL3RA encodes the alpha chain of the interleukin-3 receptor, commonly known as CD123, a cell-surface protein expressed on hematopoietic progenitors, monocytes, basophils, mast cells, plasmacytoid dendritic cells, and a subset of B cells. On its own this subunit binds IL-3 but cannot transmit a signal; only after pairing with the common beta chain shared with the GM-CSF and IL-5 receptors does the complex become competent to activate JAK2 and downstream STAT5-mediated transcription, guiding the growth and lineage commitment of blood progenitor cells. The IL3RA gene sits within a pseudoautosomal region shared by the X and Y chromosomes, alongside the related CSF2RA gene. Because CD123 is markedly upregulated on the surface of leukemic blasts in acute myeloid leukemia and related hematologic malignancies, it is widely studied as both a diagnostic marker and a candidate target for antibody-based therapies.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human IL-3Rα. During the assay, standards or samples are added to the appropriate wells together with a biotin-conjugated detection antibody also specific to Human IL-3Rα. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate. Upon addition of TMB substrate solution, only wells containing Human IL-3Rα, the biotin-conjugated detection antibody, and the avidin-HRP conjugate produce a colorimetric signal. The enzymatic reaction is stopped by the addition of a sulphuric acid stop solution, and the resulting color is measured spectrophotometrically at 450 nm (± 10 nm). Sample concentrations are then determined by comparing each well’s optical density against the generated standard curve.