Human BAFF ELISA Kit | EL-213-02
Cat. No: EL-213-02
$859.99 USD
96 wells
| Target protein | BAFF |
|---|---|
| Alternative name | TNFSF13B |
| Gene ID | 10673 |
| Reactivity | Human |
| Detection range | 78.13-5000 pg/mL |
| Sample type | serum, plasma, tissue homogenates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human BAFF/CD257 in biological samples.
Introduction
BAFF (also called BLyS or TNFSF13B) is a TNF-superfamily cytokine produced mainly by cells of the B-lymphocyte lineage. It signals through three distinct receptors – TACI, BCMA, and the B-cell-restricted BAFF receptor (BR3) – and, together with the related ligand APRIL, forms a dual-ligand, dual-receptor circuit that shapes humoral immunity. Through these receptors BAFF drives B-cell proliferation, differentiation, and long-term survival, while also contributing to T-cell costimulation and skewing of helper T-cell responses. Because excess BAFF activity is linked to abnormal B-cell persistence and autoimmune pathology, circulating BAFF levels are frequently monitored as a marker of B-cell-driven immune dysregulation.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human BAFF/CD257. During incubation, any BAFF/CD257 present in standards or samples is bound by this capture antibody, and a biotin-conjugated detection antibody directed against a second epitope on Human BAFF/CD257 is added simultaneously. Avidin conjugated to horseradish peroxidase (HRP) is then introduced and binds the biotin label. Upon addition of TMB substrate solution, a colorimetric signal develops exclusively in wells containing the BAFF/CD257–biotin-antibody–avidin-HRP complex. The enzymatic reaction is stopped by the addition of sulphuric acid, and absorbance is measured at 450 nm (± 10 nm). Sample concentrations are determined by referencing the resulting optical density values against a standard curve.