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Catalog: EL-141-PEG-hIGG Category:

Anti-PEG antibody ELISA (human IgG specific) | EL-141-PEG-hIGG

This immunogenicity assay measures the presence of human IgG in serum or plasma that bind to immobilized PEG using the direct ELISA technique.

$679.99

Description
Introduction

Polyethylene glycol (PEG) chains are often used to modify therapeutic biologic agents in order to prolong the circulating half-life of the modified protein by slowing proteolytic degradation. It has been reported that repeat injections of PEGylated proteins can induce anti-PEG antibodies. Anti-PEG antibodies can result in rapid clearance and decreased drug efficacy (accelerated blood clearance, or ABC, phenomenon).

Principal of the assay

This immunogenicity assay measures the presence of human IgG in serum or plasma that bind to immobilized PEG using the direct ELISA technique. The supplied 96 well microplate is pre-coated with PEG. After washing, quality control and test samples are pipetted into the appropriate wells. Anti-PEG antibodies bind the immobilized PEG. After washing, detection antibody (anti human IgG Peroxidase) is added. Any unbound antibody enzyme reagent is removed with a final wash and a substrate solution is added to the wells for color development. Color development is proportional to the amount of anti-PEG IgG.

Product Specifications
  • Catalog Number:
    EL-141-PEG-hIGG
  • Detection Method
    Peroxidase / OD450
  • Precision
    Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
  • Detection Limit
    62.5ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells QC samples - 6x250ul 10X assay/wash buffer - 50ml 500X biotinylated PEG(5KDa) - 50ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
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Product Specifications

  • Catalog Number:
    EL-141-PEG-hIGG
  • Detection Method
    Peroxidase / OD450
  • Precision
    Intra-assay coefficient of variation (CV) <10%. Inter-assay CV was <10%
  • Detection Limit
    62.5ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells QC samples - 6x250ul 10X assay/wash buffer - 50ml 500X biotinylated PEG(5KDa) - 50ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3

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