Human ANGPTL4 ELISA Kit | EL-213-114704
Cat. No: EL-213-114704
$859.99 USD
96 wells
| Target protein | ANGPTL4 |
|---|---|
| Gene ID | 51129 |
| Reactivity | Human |
| Detection range | 0.79-50 ng/mL |
| Sample type | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
| Sample volume | 100 µL |
| Assay time | 3h 20min |
| Precision | Intra-assay CV <8%. Inter-assay CV <10% |
| Components | Pre-Coated Microplate - 12 strips x 8 wells (4°C/-20°C) Standard (Lyophilized) - 2 vials (4°C/-20°C) Biotinylated Antibody (100×) - 120 μL (4°C/-20°C) Streptavidin-HRP (100×) - 120 μL (4°C/-20°C) Standard/Sample Diluent Buffer - 20 mL (4°C/-20°C) Biotinylated Antibody Diluent - 12 mL (4°C/-20°C) HRP Diluent - 12 mL (4°C/-20°C) Wash Buffer (25×) - 20 mL (4°C/-20°C) TMB Substrate Solution - 10 mL (4°C/-20°C (store in dark)) Stop Reagent - 6 mL (4°C/-20°C) Plate Covers - 2 pieces (RT) |
| Storage | 4°C/-20°C |
This kit employs a sandwich enzyme immunoassay technique to quantify Human ANGPTL4 in biological samples.
Introduction
ANGPTL4, also known by aliases such as PGAR and fasting-induced adipose factor, is a secreted glycoprotein produced by liver, fat tissue, and placenta whose expression rises during fasting and periods of low insulin. The protein blocks lipoprotein lipase activity, a mechanism that governs how quickly triglyceride-rich lipoproteins are cleared from the bloodstream, and this places it at the center of glucose and lipid homeostasis. Proteolytic processing separates an N-terminal coiled-coil region from a C-terminal fragment containing a fibrinogen-like domain, and the two pieces appear to have separable biological roles, including effects on endothelial cell behavior such as migration and tube formation. ANGPTL4 output climbs in endothelial cells and heart muscle cells under low-oxygen conditions, and altered circulating amounts of the protein have been linked to metabolic disturbances such as type 2 diabetes.
Principle of the Assay
This kit uses a sandwich enzyme immunoassay format in which the microtiter plate wells are pre-coated with a capture antibody specific to Human ANGPTL4. Standards or samples are dispensed into the appropriate wells alongside a biotin-conjugated detection antibody that also recognizes Human ANGPTL4. Avidin conjugated to horseradish peroxidase (HRP) is then added to each well and allowed to incubate. Upon addition of TMB substrate solution, a colorimetric signal develops exclusively in wells where Human ANGPTL4, the biotin-conjugated antibody, and avidin-HRP are all present. The enzymatic reaction is stopped by the addition of a sulphuric acid solution, and absorbance is measured spectrophotometrically at 450 nm ± 10 nm. Human ANGPTL4 concentrations in the samples are determined by referencing the measured optical densities against a standard curve.