Introduction

Adalimumab (Humira®) is a human monoclonal antibody used to block the action of tumor necrosis factor alpha (TNF-α). TNF-α is a strong proinflammatory cytokine involved in normal immunity and also various autoimmune diseases.

The Adalimumab (Humira®) ELISA kit is designed to measure free Adalimumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Adalimumab molecules in biological matrices.

Principal of the Essay

This assay employs the sandwich enzyme immunoassay technique. Anti- Adalimumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Adalimumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Adalimumab present in test samples.