From antigen design and peptide synthesis to antibody purification and labeling, AffinityImmuno’s expert staff is available to assist you with every phase of antibody development. The success of your antibody project is very important to us. As such, we encourage you to take advantage of our free Antigen Design and Protocol Review described below.

To begin discussion about any of these services, please contact us today.

Antigen Design and Protocol Review

Prior to beginning any monoclonal or polyclonal development project, you have the option of meeting with AffinityImmuno’s scientific staff for consultation on antigen design and immunization protocol. Using computer-aided technology, AffinityImmuno’s scientists will analyze peptide sequences relevant to your target antigen and review with you the predicted immunogenicity. This service is provided free of charge and aids us in selecting the appropriate immunization protocol for your antigen. While we cannot guarantee every peptide sequence will generate an immune response in the host animal, this service has proven very successful in antibody development projects.

Pricing Schedule
Antigen Design Analysis No Charge
ELISA Testing

ELISA testing can be useful in measuring and tracking the concentration of antibody in culture supernatants, bleeds, or purified materials.

Pricing Schedule
Plate Charge(up to 4 samples) $200
Antibody Purification

AffinityImmuno offers a variety of purification services to complement your antibody project. From salt fractionation to antigen-specific affinity chromatography, our expert staff will purify your antibody by any of the methods below.

Pricing Schedule
Delipidation ≤ 1 liter: $500.00

> 1 liter: Inquire

Ammonium Sulfate Fractionation ≤ 1 liter: $500.00

> 1 liter: Inquire

DEAE Chromatography ≤ 25 ml: $500.00

> 25 ml: Inquire

IgY Purification Purification of first dozen eggs: $600.00

Each additional dozen eggs: $300.00

Immunoaffinity Chromatography ≤50mL: $1400.00

>50mL to ≤100mL: $2000.00

>100mL to ≤1000mL: Inquire

Antigen Conjugation

To improve immunogenicity, certain antigens may require coupling to a larger molecule. These antigens may be coupled to KLH, BSA, or other requested proteins. Coupling methodologies include EDC and Glutaraldehyde.

Pricing Schedule
KLH Conjugation Inquire
BSA Conjugation Inquire
Alkaline Phosphatase Conjugation Inquire
Horseradish Peroxidase Conjugation Inquire
FITC Conjugation Inquire
Biotin Conjugation Inquire
Colloidal Gold Conjugation Inquire
Antibody Characterization

AffinityImmuno offers several services to help evaluate the purity, concentration, and specificity of your antibody. Please contact our Customer Support Staff for details of these services.

Pricing Schedule
BCA Total Protein Assay $300 per sample
Bradford Total Protein Assay $300 per sample
ELISA Titer Screen $50 per sample ($200 minimum plate charge)
HPLC $500 per sample
Immunoelectrophoresis $600 per plate
Radial Immunodiffusion $400 per plate (5 samples)
SDS-PAGE Reduced $500 per gel
SDS-PAGE Non-Reduced $500 per gel
Western Blot $650 up to 2 samples
Protein A Chromatography: PBS w or w/o azide ≤25mL >25mL
to
≤50mL
>50mL
to
≤100mL
>100mL
to
≤300mL
>300mL
to
≤500mL
>500mL
to
≤1000mL
Ascites $650.00 $900.00 $1300.00 $1800.00 $2200.00 $4000.00
Monoclonal Supernatant $250.00 $340.00 $500.00 $800.00 $1100.00 $1500.00
Rabbit Serum $650.00 $1000.00 $1500.00 $2000.00 $2200.00 $4000.00
Protein G Chromatography: PBS w or w/o azide ≤25mL >25mL
to
≤50mL
>50mL
to
≤100mL
>100mL
to
≤300mL
>300mL
to
≤500mL
>500mL
to
≤1000mL
Ascites $800.o0 $1000.00 $1400.00 $2000.00 $3800.00 $4000.00
Monoclonal Supernatant $300.00 $400.00 $600.00 $1000.00 $1200.00 $2000.00
Rabbit Serum $800.00 $1200.00 $1700.00 $2200.00 $4000.00 $5000.00
Goat Serum $800.00 $1200.00 $2200.00 $4000.00 $5000.00 $8000.00

All pricing is listed using a non-dedicated column. If a dedicated column is required, please inquire for pricing.

Purified antibodies can be labeled for use in various applications. Consultation with our scientific staff will assist you in selecting the reporter group and coupling chemistry best suited for your applications. Please inquire for pricing.

Reporter Group

Alkaline Phosphatase
Biotin
Colloidal Gold
Fluorescein Isothiocyanate (FITC)
Horseradish Peroxidase (HRP)