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Catalog: EL-141-201 Category:

Trastuzumab (Herceptin®) Immunogenicity ELISA | EL-141-201

This Trastuzumab (Herceptin®) Immunogenicity ELISA kit allows researchers to qualitatively monitor the presence of anti-Trastuzumab antibodies in biological matrices. Trastuzumab (Herceptin® ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2).

$809.99

Description
INTRODUCTION

Trastuzumab (Herceptin® ) is a humanized recombinant monoclonal antibody used for the treatment of primary breast cancers overexpressing human epidermal growth factor 2 (HER2). Repeated dosing of Trastuzumab has been reported to induce a neutralizing antibody response resulting in reduce efficacy of Trastuzumab. This kit allows
researchers to qualitatively monitor the presence of anti-Trastuzumab antibodies in biological matrices.

PRINCIPLE OF THE ASSAY

This immunogenicity assay employs the bridging ELISA technique. Capture antibody is precoated onto a 96 well microplate. Quality control and test samples are pipetted into the appropriate wells. Anti- Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, secondary antibody is added to the wells and after a final wash a detection reagent is added. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development.
The color development is proportional to the amount of anti-Trastuzumab present in test samples. Three levels of QC samples give a qualitative reference signal which can be used to determine the level of anti-Trastuzumab antibody in the unknown samples. The color development is stopped and the intensity of the color is measured.

Product Specifications
  • Catalog Number:
    EL-141-201
  • Application(s)
    measure anti-trastuzumab antibodies
  • Target Protein
    herceptin
  • Alternative Name of Target
    trastuzumab
  • Size
    96 wells
  • Sensitivity
    detection limit 1.5 ng/mL
  • Detection Method
    Peroxidase / OD450
  • Precision
    ntra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
  • Detection Limit
    31.3ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells QC samples - 4x50ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X secondary antibody - 17ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
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Product Specifications

  • Catalog Number:
    EL-141-201
  • Application(s)
    measure anti-trastuzumab antibodies
  • Target Protein
    herceptin
  • Alternative Name of Target
    trastuzumab
  • Size
    96 wells
  • Sensitivity
    detection limit 1.5 ng/mL
  • Detection Method
    Peroxidase / OD450
  • Precision
    ntra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
  • Detection Limit
    31.3ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells QC samples - 4x50ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X secondary antibody - 17ul 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3

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