$599.99

Trastuzumab(Herceptin®) Pharmacokinetic ELISA
Catalog EL-1611-201

Product Properties

Detection method

Peroxidase / OD450  

Storage and stability

Stable at -20°C for 1 year  

Safety Warnings and Precautions

Research Use Only. Follow instructions.  

Precision

Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. 

Detection limit

1.25ng/ml 

Each kit includes:

Coated microtiter plate, 96 wells
Calibrator diluent. - 1.8ml
Calibrator (1000 ug/mL) - 12ul
10X wash buffer - 25ml
Assay buffer - 50ml
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3  

Category:

Description

INTRODUCTION
Trastuzumab (trade name Herceptin®) is indicated for the treatment of HER2-positive breast cancer, and adjuvant therapies for metastatic gastric cancer and gastroesophageal cancer. Serum concentration of trastuzumab may predict some clinical outcome during therapy. It is also possible that the surveillance of circulating trastuzumab concentration during therapy represents a direct factor for immunogenicity and some other side effects. Identification of biomarkers and risk factors for adverse drug reactions that might be related to serum concentrations, and maintaining the effective minimum concentration of trastuzumab in order to potentially avoid some side effects, might be beneficial using a reliable method.
The Trastuzumab ELISA kit is designed to measure trastuzumab with high specificity and enhanced sensitivity. The assay design utilizes a pair of antibodies that allows the detection of the whole trastuzumab molecule in biological matrices.
PRINCIPLE OF THE ASSAY
This assay employs the sandwich enzyme immunoassay technique. Anti-Trastuzumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Trastuzumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Trastuzumab present in test samples.

 

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Catalog EL-1611-201”

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