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Catalog: EL-1611-161 Category:

Pembrolizumab(Keytruda®) Pharmacokinetic ELISA | EL-1611-161

Pembrolizumab ELISA Keytruda ELISA Biosimilars. The Pembrolizumab ELISA kit is designed to measure free Pembrolizumab with high specificity and sensitivity.

$679.99

Description
INTRODUCTION

Pembrolizumab (Trade name Keytruda®) is a human monoclonal antibody used to block the action of Programmed Cell Death Protein 1(PD-1). Upregulation of PD-1 ligands on tumor cells can allow the tumor to evade anti-tumor immune response. By blocking PDL1 and PD-L2 binding to PD-1, Pembrolizumab induces an anti-tumor immune response by allowing full activation of the anti-tumor T cell response. Thus, Pembrolizumab acts as a checkpoint inhibitor to stimulate immune responses to eliminate cancer cells.

The Pembrolizumab ELISA kit is designed to measure free Pembrolizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Pembrolizumab molecules in biological matrices.

PRINCIPLE OF THE ASSAY

This assay employs the sandwich enzyme immunoassay technique. Standards and test samples are pipetted into the appropriate wells. Pembrolizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody enzyme reagent and a substrate solution is added to the wells for color development. The color development is proportional to the amount of Pembrolizumab present in test samples.

 

Product Specifications
  • Catalog Number:
    EL-1611-161
  • Application(s)
    Measure free Trastuzumab
  • Target Protein
    keytruda
  • Alternative Name of Target
    pembrolizumab
  • Size
    96 wells
  • Sensitivity
    detection limit 1.5 ng/mL
  • Detection Method
    Peroxidase / OD450
  • Precision
    Inter-assay CV <10%.
  • Detection Limit
    0.39ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (500 ug/mL) - 12ul 10X wash buffer - 50ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
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Product Specifications

  • Catalog Number:
    EL-1611-161
  • Application(s)
    Measure free Trastuzumab
  • Target Protein
    keytruda
  • Alternative Name of Target
    pembrolizumab
  • Size
    96 wells
  • Sensitivity
    detection limit 1.5 ng/mL
  • Detection Method
    Peroxidase / OD450
  • Precision
    Inter-assay CV <10%.
  • Detection Limit
    0.39ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (500 ug/mL) - 12ul 10X wash buffer - 50ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3

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