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Catalog: EL-1611-021 Category:

Bevacizumab(Avastin®) Pharmacokinetic ELISA | EL-1611-021

The Bevacizumab ELISA kit is designed to measure free Bevacizumab with high specificity and sensitivity, through use of a pair of antibodies.

$679.99

Description
INTRODUCTION

The Bevacizumab ELISA kit is designed to measure free Bevacizumab with high specificity and sensitivity. The assay design uses a pair of antibodies allowing detection of whole Bevacizumab molecules in biological matrices. Bevacizumab (Trade name Avastin®) is a recombinant humanized monoclonal antibody that blocks angiogenesis by inhibiting vascular endothelial growth factor A(VEGF-A). VEGF-A is a chemical signal that stimulates angiogenesis in a variety of diseases, especially in cancer. Bevacizumab was the first clinically available angiogenesis inhibitor in the United States.

PRINCIPLE OF THE ASSAY

This assay employs the sandwich enzyme immunoassay technique. Anti-Bevacizumab is coated onto a 96 well microplate. Calibrator, quality control samples and test samples are pipetted into the appropriate wells. Bevacizumab present in biological matrices is bound by the immobilized capture antibody. After washing away any unbound substances, enzyme linked detection antibody is added to the wells. The plate is washed to remove any unbound antibody-enzyme reagent and a substrate solution is added to the wells for color development. The color devel opment is proportional to the amount of Bevaciuzumab present in test samples.

Product Specifications
  • Catalog Number:
    EL-1611-021
  • Application(s)
    measure free Bevacizumab
  • Target Protein
    avastin
  • Alternative Name of Target
    bevacizumab
  • Size
    96 wells
  • Sensitivity
    detection limit 1.5 ng/mL
  • Detection Method
    Peroxidase / OD450
  • Precision
    Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
  • Detection Limit
    1.5ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3
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Product Specifications

  • Catalog Number:
    EL-1611-021
  • Application(s)
    measure free Bevacizumab
  • Target Protein
    avastin
  • Alternative Name of Target
    bevacizumab
  • Size
    96 wells
  • Sensitivity
    detection limit 1.5 ng/mL
  • Detection Method
    Peroxidase / OD450
  • Precision
    Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%.
  • Detection Limit
    1.5ng/ml
  • Storage Conditions
    Stable at -20°C for 1 year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
  • Each Kit Includes
    Coated microtiter plate, 96 wells Calibrator diluent. - 1.8ml Calibrator (250 ug/mL) - 12ul 10X wash buffer - 25ml Assay buffer - 50ml 1000X detection reagent - 17ul TMB - 12ml TMB stop solution - 12ml Plate sealers - 3

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