$599.99

Anti PEG antibody ELISA (human IgG specific)
Catalog EL-141-PEG-hIGG

Product Properties

Detection method

Peroxidase / OD450 

Storage and stability

Stable at -20°C for 1 year 

Safety Warnings and Precautions

Research Use Only. Follow instructions. 

Precision

Intra-assay coefficient of variation (CV) <10%. Inter-assay CV <10%. 

Detection limit

62.5ng/ml 

Each kit includes:

Coated microtiter plate, 96 wells
QC samples - 6x250ul
10X assay/wash buffer - 50ml
500X biotinylated PEG(5KDa) - 50ul
1000X detection reagent - 17ul
TMB - 12ml
TMB stop solution - 12ml
Plate sealers - 3 

Category:

Description

INTRODUCTION
Polyethylene glycol (PEG) chains are often used to modify therapeutic biologic agents in order to prolong the circulating half-life of the modified protein by slowing proteolytic degradation. It has been reported that repeat injections of PEGylated proteins can induce anti-PEG antibodies. Anti-PEG antibodies can result in rapid clearance and decreased drug efficacy (accelerated blood clearance, or ABC, phenomenon).
PRINCIPLE OF THE ASSAY
This immunogenicity assay measures the presence of human IgG in serum or plasma that bind to immobilized PEG using the direct ELISA technique. The supplied stretavidin precoated 96 well microplate is incubated with biotinylated PEG (5KDa). After washing, quality control and test samples are pipetted into the appropriate wells. Anti-PEG antibodies bind the immobilized PEG. After washing, detection antibody (anti human IgG Peroxidase) is added. Any unbound antibody enzyme reagent is removed with a final wash and a substrate solution is added to the wells for color development. Color development is proportional to the amount of anti-PEG IgG.

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Catalog EL-141-PEG-hIGG”

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