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Catalog: ab-131-295-PAIR Categories: , ,

5 Kits - COVID-19 Spike Capture and Detection (HRP conjugate) ELISA pair with calibrator | ab-131-295-PAIR

This product is a SARS-CoV-2 (COVID-19, 2019-nCOV) Spike S2 domain Antibody. host species – chicken, raised against recombinant SARS-CoV-2 (COVID-19).

$800.00

Description

Antibody pair for SARS-CoV-2 Spike protein (COVID-19, 2019-nCoV). Capture is rabbit anti-S1 spike protein domain. Detection is HRP-conjugate of chicken anti-S2 spike protein domain.

Sufficient material for: 5 X 96 well plates.

Catalog ab-131-295-PAIR


 

 

Figure 1: ELISA test COVID-19 spike protein using antibody pair ab-131-295-PAIR. Antibodies: Rabbit anti-spike protein S1 was coated at a dilution of 1/1000 in PBS for 1 hour at room temperature. After blocking, calibrator was diluted from 2000ng/ml to 15ng/m1 in blocking buffer and incubated for 1 hour on coated plate. After washing detection antibody (Chicken anti-spike protein S2 – HRP) was diluted 1/1000 in blocking buffer and incubated on the calibrator wells for 1 hour. After a final wash and tamping, the ELISA was developed by the addition of 100u1TMB to each well and stopped after 15 minutes by the addition of 0.2M sulfuric acid.

 

SPECIFICATIONS

  • HOST SPECIES: Capture: Rabbit – Detection: Chicken
  • SPECIES REACTIVITY: SARS-CoV-2 spike protein.
  • HOMOLOGY: Predicted reactivity based on immunogen sequence: SARS-CoV Spike protein (52): (100%)
  • IMMUNOGEN: Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Capture antibody was raised against recombinant SARS-CoV-2 (2019- nCoV) spike protein (S1) domain. Detection antibody was raised against recombinant SARS-CoV-2 (2019- nCoV) spike protein (S2) domain.

 

TESTED APPLICATIONS: ELISA

Calibrator supplied: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein (17uL©250ug/m1). Full length extracellular domain with C terminal HIS affinity tag. Produced by over expression in insect cell line and binding the ACE2 confirmed by ligand binding assay.

HANDLING INFORMATION

  • Anti-S1 (Capture) formulation:  Sterile filtered in PBS. No preservatives
  • Anti-S2-HRP (Detection) formulation: Sterile filtered in PBS. No preservatives
  • Calibrator (Spike protein) formulation: 20mM Tris, 300mM NaCI, 10% Glycerol, 5% Trehalose, 5% Mannitol, 0.01% Tween-80. pH 8.0

 

 

Product Specifications
  • Catalog Number:
    ab-131-295-PAIR
  • Target Protein
    COVID
  • Alternative Name of Target
    SARS-CoV-2
  • Size
    96 wells
  • Clonality
    polyclonal
  • Isotype
    IgY
  • Host Specie(s)
    capture - rabbit, detection - chicken
  • Storage Conditions
    3 months and -20°C for one year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.
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Product Specifications

  • Catalog Number:
    ab-131-295-PAIR
  • Target Protein
    COVID
  • Alternative Name of Target
    SARS-CoV-2
  • Size
    96 wells
  • Clonality
    polyclonal
  • Isotype
    IgY
  • Host Specie(s)
    capture - rabbit, detection - chicken
  • Storage Conditions
    3 months and -20°C for one year
  • Safety Warnings and Precautions
    Research Use Only. Follow instructions.

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