Figure 1: ELISA test COVID-19 spike protein using antibody pair ab-131-295-PAIR. Antibodies: Rabbit anti-spike protein S1 was coated at a dilution of 1/1000 in PBS for 1 hour at room temperature. After blocking, calibrator was diluted from 2000ng/ml to 15ng/m1 in blocking buffer and incubated for 1 hour on coated plate. After washing detection antibody (Chicken anti-spike protein S2 – HRP) was diluted 1/1000 in blocking buffer and incubated on the calibrator wells for 1 hour. After a final wash and tamping, the ELISA was developed by the addition of 100u1TMB to each well and stopped after 15 minutes by the addition of 0.2M sulfuric acid.
Calibrator supplied: SARS-CoV-2 (COVID-19, 2019-nCoV) Spike protein (17uL©250ug/m1). Full length extracellular domain with C terminal HIS affinity tag. Produced by over expression in insect cell line and binding the ACE2 confirmed by ligand binding assay.
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